Abstract
C6orf141 (Chromosome 6 open reading frame 141) is a novel gene, and its role in oral cancer progression remains unclear. C6orf141 expression in oral squamous cell carcinoma (OSCC) and adjacent normal tissues from 428 patients was examined through immunohistochemistry (IHC). Our results revealed that C6orf141 expression was significantly reduced in OSCC compared with adjacent normal tissues. Low C6orf141 expression was significantly associated with a poor American Joint Committee on Cancer pathological stage (P < 0.001), T classification (P = 0.002), and pN stage (P = 0.032). Kaplan–Meier curves revealed that low C6orf141 expression was significantly associated with shorter disease-specific survival (DSS) in patients with OSCC (log-rank P = 0.007). Multivariate analysis indicated that low C6orf141 expression was an independent prognostic biomarker for DSS (adjusted hazard ratio = 1.34; 95% confidence interval = 1.10–1.81; P = 0.05). Additionally, ectopic C6orf141 expression could significantly suppress oral cancer cell proliferation, colony formation, and migratory and invasive abilities. Xenograft tumor growth assay revealed that C6orf141 could significantly suppress oral tumor growth in vivo. Our results suggest that C6orf141 plays a novel tumor-suppressive role in oral cancer cell growth and motility. Furthermore, C6orf141 dysfunction could be a potential prognostic biomarker for OSCC and provide new therapeutic strategies in the future.
Highlights
Our previous study, we identified several deregulated genes in oral cancer through a next-generation sequencing approach[10]
Our data revealed that the expression levels of the protein-coding transcripts of C6orf[141] were significantly reduced, whereas those of noncoding transcripts had no significant difference in oral squamous cell carcinoma (OSCC) compared with adjacent normal tissue (Fig. 1b,c)
Of the 428 patients with OSCC examined in this study, 183 had buccal mucosa SCC (BMSCC) and 245 had tongue SCC (TSCC)
Summary
Our previous study, we identified several deregulated genes in oral cancer through a next-generation sequencing approach[10]. Among these dysfunctional genes, chromosome 6 open reading frame 141 (C6orf141) located at chromosome 6p12.3 was identified as being involved in deregulating OSCC. The role of C6orf[141] in human cancer is unknown. The clinical effect of C6orf[141] expression on OSCC remains largely unknown, and details of its role has not yet been fully elucidated. We assessed the association between C6orf[141] expression and clinical pathological features. We assessed that effect of C6orf[141] expression on cell growth and the invasion of OSCC cell lines
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
