Abstract
A Love wave acoustic device has been utilized for monitoring antibody-antigen immunoreactions in aqueous solutions in real time. High sensitivity and selectivity are achieved. In this study, sheep IgG is immobilized on the device surface as a receptor layer for detection of antisheep IgG in buffer solution. The kinetics of the antibody-antigen binding are investigated by monitoring the frequency change against reaction time for a wide range of antibody concentrations. A general analytic expression for the frequency against time and antibody concentrations is derived. A response to a concentration of 1 ng ml −1 is unambiguously detected. A dual-channel delay-line configuration. with one channel for sensing and the other as reference, is used to give a comparison of specific and non-specific responses as well as to reduce the temperature effects. The Love wave sensor is robust, reliable and reusable many times.
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