Abstract

Abstract—Loss of photoreactivability during liquid‐holding occurs much faster in Escherichia coli WP2 than has been reported for another strain of B/r (Harm, 1968). The underlying mechanism considerably reduces the maximum photoreactivation attainable within 30 min. The addition of caffeine to UV irradiated strain WP2 prevents the loss of photoreactivation activity. Similarly, a uvrA mutant also fails to show this loss of photoreactivation capacity. These results are consistent with the view that in WP2 the initial steps of excision repair take place during liquid‐holding and that they can preclude photoreactivation at a high rate. But it remains to be elucidated why different strains of B/r differ with respect to this rate.

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