Abstract
Some insect viruses produce the occlusion body (OB), a large crystalline particle comprising a viral protein that occludes virions to protect them from harsh environments. The shapes and sizes of OBs are diverse depending on baculovirus species, but the detailed molecular mechanism determining them has yet to be totally clarified yet. Here we generated Bombyx mori nucleopolyhedrovirus (BmNPV) mutants of the p24 gene that encodes a viral capsid protein and found that p24-mutated BmNPVs produced cuboidal OBs with a slightly larger size than typical truncated octahedral OBs produced by wild-type BmNPVs. Meanwhile, p24 disruption has no significant impact on progeny virus production and viral pathogenicity. In addition, we experimentally demonstrated that a single amino acid substitution found in the P24 protein of the BmNPV Cubic isolate caused cuboidal OB production. These results suggest that p24 has a crucial role in generating the typical shape of OBs.
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