Loss of Genetic Diversity in Hatchery Produced Haliotis Diversicolor Supertexta by AFLP

Abstract

Information on genetic diversity and differentiation is essential for conservation and stock improvement programs. AFLP technique was applied to analyze the genetic diversity and differentiation of one wild and three hatchery Haliotis diversicolor supertexta populations. In general, our results showed a reduced genetic diversity in the three hatchery populations when compared with the one wild population. The results showed that in three hatchery populations, the percentage of polymorphic loci ranged from 42.08% to 51.91%, the mean number of alleles per locus ranged from 1.4208 to 1.5191, the mean effective number of alleles per locus ranged from 1.2790 to 1.3547, Neipsilas gene diversity ranged from 0.1633 to 0.2044, and the Shannonpsilas information index ranged from 0.2414 to 0.3002. While in the wild population, the percentage of polymorphic loci was 61.48%, the mean number of alleles per locus was 1.6148, the mean effective number of alleles per locus was 1.4233, Neipsilas gene diversity was 0.2450, and the Shannonpsilas information index was 0.3598. The genetic differentiation among the four populations was low. The genetic distance between the wild NA population and other three hatchery populations ranged from 0.0372 to 0.0556.