Loss of Culturability of Salmonella enterica subsp. enterica Serovar Typhimurium upon Cell-Cell Contact with Human Fecal Bacteria

Abstract

Loss of culturability of Salmonella enterica subsp. enterica serovar Typhimurium has been observed in mixed cultures with anaerobic fecal bacteria under conditions that allow local interaction between cells, such as cell contact. A reduction of a population of culturable S. Typhimurium on the order of ∼10(4) to 10(5) CFU/ml was observed in batch anaerobic mixed cultures with fecal samples from different human donors. Culturability was not affected either in supernatants collected at several times from fecal cultures, when separated from fecal bacteria by a membrane of 0.45-μm pore size, or when in contact with inactivated fecal bacterial cells. Loss of culturability kinetics was characterized by a sharp reduction of several logarithmic units followed by a pronounced tail. A mathematical model was developed to describe the rate of loss of culturability as a function of the frequency of encounters between populations and the probability of inactivation after encounter. The model term F(S · F)(1/2) quantifies the effect of the concentration of both populations, fecal bacteria (F) and S. Typhimurium (S), on the loss of culturability of S. Typhimurium by cell contact with fecal bacteria. When the value of F(S · F)(1/2) decreased below ca. 10(15) (CFU/ml)(2), the frequency of encounters sharply decreased, leading to the deceleration of the inactivation rate and to the tailing off of the S. Typhimurium population. The probability of inactivation after encounter, P, was constant, with an estimated value of ∼10(-5) for all data sets. P might be characteristic of the mechanism of growth inhibition after a cell encounter.