Loss of core fucosylation suppressed the humoral immune response in Salmonella typhimurium infected mice.

Abstract

The humoral immune response is pivotal to protect the host fromSalmonella typhimurium(S. typhimurium) infection. Previously, we found that core fucosylation catalyzed by core fucosyltransferase (Fut8) could regulate the immune responses. However, the role of core fucosylation duringS. typhimuriuminfection remains unclear. To demonstrate the role of Fut8 inS. typhimuriuminfection, we infected Fut8+/+ and Fut8-/-mice usingS. typhimurium. The production of antiserum against the S. typhimurium was detected. The expression of T and B cell activation-related genes during S. typhimurium infection was analyzed. The role of core fucosylation on CD4+ T-B cell interaction and B cell generation was investigated during S. typhimurium infection. The production of sIgA was compared between Fut8+/+and Fut8-/-mice. Compared to Fut8+/+mice, the number ofS. typhimuriumcolonized in the cecumwas markedly increased in Fut8-/- mice. The production of the IgG and sIgA specificforS. typhimuriumwas significantly decreased in Fut8-/-mice. Moreover, loss of Fut8 decreased the induction of Th2-type cytokines from splenic cells of Fut8-/-mice duringS. typhimuriuminfection. In addition, we found that the core fucosylation regulated the interaction between B and T cells in the lipid raft formation. Core fucosylation plays important roles in host defence against S.typhimurium infection.