Abstract
Publisher Summary This chapter discusses a study to investigate the loss of conditioning by perforant path stimulation after the local microinjection of colchicine in dentate area. In this study, 20 male Wistar rats were used. Bipolar stimulating electrodes were implanted in the perforant path and chemitrodes for recording and application of colchicine into the stratum moleculare of the ipsilateral dentate area. After 8–10 days, that is, after having obtained the input/output curves, 1.6 μg colchicine in 0.2 μl physiological saline or 0.2 μl saline alone was injected. After 30 days, shuttle-box training was started. It was found that ipsilateral intrahippocampal injection of 1.6 μg colchicine destroys the granule cells. After saline injection alone, no changes could be detected. Alterations in the contralateral hippocampus were neither observed lightmicroscopically after colchicines nor after saline injection. Previously registered field potentials in the dentate gyrus evoked by perforant path stimulation disappear after colchicine treatment. The colchicine-induced destruction of granule cells in dentate area prevents conditioning by perforant path stimulation as conditioned stimulus.
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