Abstract
Since considerable, although variable, amounts of absorbed lipid are lost from intestinal epithelium during processing for electron microscopy, the following experiments were carried out to evaluate this loss during various, relatively new processing procedures.Everted sacs of rat jejunum were incubated in a micellar solution of oleic acid-3H (1.0 mM), monoolein (0.5 mM), and sodium taurocholate (15.0 mM) for 30 min at 37°C. Samples of these everted sacs were processed for electron microscopy by four different procedures which are outlined in TABLE I. The radioactivity lost to the processing solutions and remaining in the infiltrated tissue was monitored by liquid scintillation counting.Routine Processing included a single OSO4 fixation followed by ethanol (EtOH) dehydration, propylene oxide (PO) and Epon infiltration. The total loss of 3H-lipid was 67% of the total absorbed oleic acid-3H (TABLE II).
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: Proceedings, annual meeting, Electron Microscopy Society of America
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
