Los polimorfismos de ITPA no predicen un riesgo adicional más allá de TPMT y NUDT15 para citopenia inducida por tiopurina en la enfermedad inflamatoria intestinal

Abstract

Introduction and aimThiopurine-related leukopenia is associated with polymorphisms in the thiopurine methyltransferase (TPMT) and nucleoside diphosphate-linked moiety X type motif 15 (NUDT15) genes. However, those polymorphisms explain only a fraction of thiopurine-related leukopenia. Our aim was to study the role of an inosine triphosphate pyrophosphatase (ITPA) polymorphism in patients with inflammatory bowel disease (IBD) and thiopurine-related leukopenia that was unexplained by the TPMT and NUDT15 polymorphisms. Material and methodsWe enrolled consecutive IBD patients on thiopurines (azathioprine or 6-mercaptopurine) from January 2019-March 2020, at a tertiary care center in North India. The presence of the ITPA (C.94C>A) polymorphism was evaluated in all patients, along with its association with thiopurine-related leukopenia. ResultsOf the 33 patients (from a total of 119 patients) that developed leukopenia, 8 had the TPMT (n=1) or NUDT15 (n=7) polymorphism. Of the remaining 111 patients, their mean age was 36.36±13.54 years and 57 (51.3%) were males. Twenty-five (21.01%) had unexplained leukopenia. The ITPA polymorphism was detected in 4 (16%) patients in the unexplained leukopenia group and 24 (27.9%) patients in the non-leukopenia group (P=.228). The odds ratio for predicting leukopenia with the ITPA polymorphism was 0.4921 (95% CI: 0.1520-1.5830; P=.234). ConclusionThe ITPA (C.94C>A) polymorphism was frequently detected in the study population but was not predictive for leukopenia in patients with IBD on thiopurine therapy.