Abstract
Loop-mediated isothermal amplification ( LAMP ) , a novel isothermal nucleic acid amplification method, employs a DNA polymerase and four specially designed primers that recognize a total of six distinct sequences on the target DNA to amplify the target via stem-loop DNA structure. LAMP allows the synthesis of large amounts of DNA in a short period of time with high specificity under isothermal conditions. As the LAMP reaction progresses, the reaction by-product pyrophosphate ions bind to magnesium ions and form a white precipitate of magnesium pyrophosphate. Real-time turbidity measurements of the LAMP reaction make it to be a very useful molecular method for quantitutire analysis of nucleic acid. Key words: Nucleic acid amplification techniques; DNA viruses; RNA viruses
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