Long-Term Potentiation Enhanced in Juvenile Rat by Repetitive Cortical Spreading Depression

Abstract

Introduction Spreading depression )SD( is a bioelectrical event in the central nervous system and involves in the pathophysiology of some neurological disorders. In this present study, we indicate enhancement of long-term potentiation of hippocampus tissue in juvenile rats faced to cortical spreading depression repetitively. Materials and Methods Silver recording electrodes as well as a cannula were implanted over the brain of juvenile rats. Repetitive cortical SD events were induced by KCl 3 M weekly injection through the cannula. The brains were removed after 4 weeks. Transverse sections were prepared and incubated in artificial cerebrospinal fluid. Single electrical stimulations were applied through a bipolar electrode placed on to the hippocampal Schaffer collaterals. The field excitatory postsynaptic potentials (fEPSP) were elicited by adjusting the intensity of stimulation to 50% of that at which population spikes began to appear. Results Repetitive SD enhanced the long-term potentiation in CA1 hippocampal area. The data indicate that repetitive cortical SD in juvenile rats significantly increases the amplitude of the fEPSP form the baseline. Conclusion This may clarify the pathophysiology of memory deficit were seen in some neurological disorders in children. ناوجون یاه شوم رد يرشق ي هدنوش رشتنم راهم رد پمکوپیه تدم ینلاوط یسپانیس تیوقت شیازفا Long-Term Potentiation Enhanced in Juvenile Rat by Repetitive Cortical Spreading Depression ،1یوسوم سردم یفطصم دیس ،1يتشدرد هدازانب زانرهم ،1، 2یدمحا دلایم 1، 3هداز میرک ابیرف .ناریا ،نارهت ،ءایبنلاا متاخ ناتسرامیب ،افش باصعا مولع تاقیقحتزکرم .1 .ناریا ،جرک ،يملاسا دازآ هاگشناد ،جرک دحاو ،يكشزپماد هدكشناد .2 .ناریا ،نارهت ،ناریا یكشزپ مولع هاگشناد ،يكشزپ نیون ياه يروآ نف هدكشناد .3 Milad Ahmadi1, 2 , Mehrnaz Banazadeh Dardashti1, Sayed Mostafa Modarres Mousavi1, Fariba Karimzadeh1, 3 1. Shefa Neuroscience Research Center, Khatam-al-Anbia Hospital, Tehran, Iran. 2. Faculty of Verterinary Medicine, Karaj Branch, Islamic Azad University, Karaj, Iran. 3.School of Advanced Technologies in Medicine, Iran University of Medical Sciences, Tehran, Iran. :هژاو دیلک ،تدم ينلاوط يسپانیس تیوقت ،يریگدای ،هظفاح ،ينورون هتیسیتسلاپ .پمکوپیه ه ديكچ