Abstract
Living cells dynamicallychange their morphology and function accordingto the cell cycle. Long-term observations of living cells are privileged whenwespy the unique, cell cycle-driven molecularevents, whichcannot be obtained from short-term ones. Mg2+, a metal ion abundant in cells, has been shown to be involved in a variety of physiological phenomena by noninvasive cellular observation using fluorescence microscopy. However, long-term observation of Mg2+ in cells has been a great challenge. Herein, we present a protocol for the long-term microscopic imaging of intracellular Mg2+ levels using a small molecule-protein hybrid fluorescent probe we developed.
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