Abstract
Objectives:The aim of the present study was to investigate the effects of root canal sealers on the cytotoxicity of 3T3 fibroblasts during a period of 5 weeks.Material and Methods:Fibroblasts (3T3, 1x105 cells per well) were incubated with elutes of fresh specimens from eight root canal sealers (AH Plus, Epiphany, Endomethasone N, EndoReZ, MTA Fillapex, Pulp Canal Sealer EWT, RoekoSeal and Sealapex) and with elutes of the same specimens for 5 succeeding weeks after immersing in simulated body fluid. The cytotoxicity of all root canal sealers was determined using the MTT assay. Data were analyzed using ANOVA and Tukey's test.Results:RoekoSeal was the only sealer that did not show any cytotoxic effects (p<0.05). All the other tested sealers exhibited severe toxicity initially (week 0). MTA Fillapex remained moderately cytotoxic after the end of experimental period. Toxicity of the other tested sealers decreased gradually over time. The evaluated root canal sealers presented varying degrees of cytotoxicity, mainly in fresh mode.Conclusions:RoekoSeal had no cytotoxic effect both freshly mixed and in the other tested time points. MTA Fillapex was associated with significantly less cell viability when compared to the other tested root canal sealers.
Highlights
A complete sealing of the root canal system after cleaning and shaping is critical for a successful endodontic treatment20
They are often placed in intimate contact with the periapical tissues for an extended period of time12,16
RoekoSeal was the only sealer that did not show any cytotoxic effects in any WLPH SRLQW DQG ZDV QRW VLJQL¿FDQWO\ GLIIHUHQW IURP the control (p
Summary
A complete sealing of the root canal system after cleaning and shaping is critical for a successful endodontic treatment. Endodontic sealers are designed to be used only within the root canal during endodontic therapy, sometimes they can extrude through the apical constriction. Endodontic sealers are designed to be used only within the root canal during endodontic therapy, sometimes they can extrude through the apical constriction13,16 They are often placed in intimate contact with the periapical tissues for an extended period of time. The currently used sealers are based on zinc oxideeugenol, calcium hydroxide, polydimethylsiloxane, silicone, epoxy resin and methacrylate resin They exhibit a variable degree of cytotoxicity depending on the conditions under which testing was performed. They exhibit a variable degree of cytotoxicity depending on the conditions under which testing was performed5,6 Most of these sealers exert some toxic effect when they are fresh or in short testing times. A few studies have attempted to evaluate the longitudinal cytotoxicity effects of root canal sealers
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