Abstract
A recombinant DNA CHO cell line secreting urokinase-type plasminogen activitor (uPA) was cultivated with Cytopore cellulose porous microcarriers in a 20L Biostat UC20 stirred tank reactor The reactor was equipped with bubble-free silicone tubing oxygenator, and a modified perfusion system which can be washed-out backward was substitute for spin-filter to prevent filter clogging During 100 days culture with 0 1% fetal bovine serum (FBS) medium the maximal cell density was over 107/ml, the maximal uPA activity in supernatant reached 6250 IU/ml, and 1604L supernatant contained about 51guPA(about70%was single chain form) was harvested. Recombinant CHO cells can move from seed porous microcarriers occupied by rCHO cells to vacant microcarriers spontaneously without trypsinization andcontinue to grow until all the microcarriers contained rCHO cells It shows that Cytopore porous miaocarriers is very useful and convenient for enlarging cell culture scale step by step
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