Long-lasting excitation of protocerebral bilateral neurons in the pheromone-processing pathways of the male moth Bombyx mori

Abstract

Intracellular recording and staining with Lucifer yellow were used to characterize the responses and structure of pheromone-processing bilateral neurons in the protocerebrum of the brain of the male silkworm moth Bombyx mori. Numerous olfactory bilateral neurons innervated a particular neuropil region lateral to the central body, the lateral accessory lobe (LAL). The LALs are linked to each other by bilateral neurons with arborizations in each LAL. The LAL appears to be important for collecting the olfactory information from both sides of the brain. Many of the bilateral neurons showed a characteristic long-lasting excitation (LLE) that outlasted the olfactory stimuli (1.5 s). In some preparations, the LLE lasted more than 20 s and the firing gradually decreased to the background level.