Abstract
Gold nanorod-based affinity sensors were developed utilizing concanavalin-A/dextran/glucose chemistry to detect glucose. The gold nanorods, synthesized using the seed-mediated method, were first coated with dextran sulfate. The dextran sulfate coating changed the local refractive index, which was observed as a red shift in the longitudinal plasmon band. After the addition of 8 μM concanavalin-A, gold nanorod aggregates were formed as shown by TEM analysis. The corresponding UV-Vis spectrum peak wavelength showed a red shift from 701 nm of dextran sulfate coated gold nanorods to 718 nm of concanavalin-A promoted gold nanorod aggregates. The gold nanorod aggregates were dissociated when 12 mM glucose was introduced, as illustrated by TEM images. The longitudinal plasmon resonance of the UV-Vis spectrum also showed a blue shift from 718 nm of dextran sulfate-coated gold nanorods associated by concanavalin-A to 714 nm of dissociated dextran sulfate-coated gold nanorods. The degree of the spectrum peak wavelength shift of the dextran sulfate-coated gold nanorods associated by concanavalin-A can be modulated using glucose ranging from 1 to 30 mM.
Highlights
Noble nanoparticles have diverse uses in a plethora of applications [1,2,3,4]
Our results showed that dextran sulfate-coated gold nanorods (GNRs) aggregates were formed by introducing 8 μM ConA, as illustrated by TEM images
Since the presence of CTAB could affect the detection of glucose [20], negatively charged dextran sulfate was used to cover CTAB-stabilized GNRs using electrostatic force
Summary
Noble nanoparticles have diverse uses in a plethora of applications [1,2,3,4]. As such, it is no wonder that gold nanoparticles are utilized in a myriad of applications related to biotechnology and nanomedicine [5,6]. The unique interaction between light and gold nanoparticles makes them attractive materials for sensing applications The results of these interactions, including surface plasmon resonance, absorption, scattering or total extinction, are dependent on the size, shape, structure, dielectric properties and surrounding medium of the metal [7,8]. Glucose, which has a higher affinity to ConA than dextran, breaks the aggregates of gold nanoparticles This leads to a change in the local refractive index as well as absorbance. The longitudinal plasmon resonance of UVVis spectrum revealed a blue shift from 718 nm of ConA trigged GNR aggregates to 714 nm of dissociated dextran sulfate-coated GNRs
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