Abstract
Liposomes are widely used as carriers of drugs, genes, or contrast agents while albumin is a commonly used tracer for permeability studies. Here we studied the permeability and distribution of 64Cu‐labeled liposomes and albumin (64Cu‐BAT‐liposomes, Seo et al. Bioconjugate Chem.19: 2577, 2008, and 64Cu‐BAT‐BSA) in a mouse model of ductal carcinoma in situ. Precancer mammary intraepithelial neoplasia outgrowth (MINO, line 4) tissue was transplanted bi‐laterally into gland cleared mammary fat pads in young FVB/N mice (n=8) and were PET scanned (Focus 120, Siemens Medical Solutions, Inc.) at week 3, 5, 7 and 8 after transplantation. Each animal was scanned at multiple time‐points after bolus injection of the tracer to create time‐activity curves (TACs). The apparent permeability of both tracers increased over time as the lesions transformed, and was higher for albumin than for liposomes (from 3.46 ± 0.69 to 12.48 ± 2.94 and 0.81 ± 0.61 to 2.29 ± 0.56 10−8 cm/s for albumin and liposomes at week 3 and 8, respectively). In general, the distribution of liposomes within the tumor was more heterogeneous than with albumin.In conclusion, longitudinal progression of disease could be detected in vivo with PET imaging, as well as heterogeneity of tracer permeability and distribution within the lesions which is valuable information when evaluating drug‐delivery efficacy.This project was supported by NIH CA 103828 and CA 134659
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