Longitudinal elongation of primary afferent axons in the dorsal funiculus of the chick embryo spinal cord

Abstract

The longitudinal elongation of primary afferent axons (PAAs) in the dorsal funiculus of chick embryo spinal cord was examined using a lipophilic tracer, DiI and immunohistochemistry. The earliest developing PAAs in the brachial segments invaded the spinal cord around embryonic day (E) 3.5. Thereafter, they elongated both rostrally and caudally in the presumptive dorsal funiculus, with frequent contacts between pre-existing axons and later arriving growth cones. By E4, the PAAs had elongated 3 segments both rostrally and caudally. In the course of their longitudinal elongation, the PAAs shifted their trajectory dorsally within the dorsal funiculus. By E6–6.5, the PAAs had extended as far as 10 segments rostrally and 6 segments caudally in the dorsal funiculus, and collaterals began to enter the dorsal horn. By E9, the PAAs extended up to 13 segments rostrally and 7 segments caudally, and collaterals reached the ventral spinal cord. During their longitudinal course, the PAAs shifted their trajectory medially within the dorsal funiculus.