Abstract
Generating an expected longitudinal pH distribution is of essential importance for achieving high resolution in an electrophoresis process conducted in a multicompartment electrolyzer. A simulation of the longitudinal distribution of pH in a multicompartment electrolyzer is proposed on the basis of mass transfer analysis and electrolysis equilibrium of the electrolytes. Selection of the operating conditions to establish an expected pH distribution with a conventional electrolyte rather than an ampholyte can thus be accomplished according to the simulation results. Numerical solution of this model is developed on the basis of the Gear method. The calculated results agree well with the experimental data in a wide range of operation conditions including applied potential, sample loading flow rate, and protein concentration in solution. This model may also be applied to other ampholyte-free electrophoresis processes conducted in a membrane-spaced multicompartment electrolyzer.
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