LONGITUDINAL CHARACTERIZATION OF LYMPHOCYTES OBTAINED FROM PREGNACIES AS LOW OR HIGH RISK OF PREECLAMPSIA

Abstract

Event Abstract Back to Event LONGITUDINAL CHARACTERIZATION OF LYMPHOCYTES OBTAINED FROM PREGNACIES AS LOW OR HIGH RISK OF PREECLAMPSIA Fabián I. Tempio Sepúlveda1, 2, 3, Cristina Rivera Arcos1, 2, Gustavo A. Caro Díaz1, 2, Mauro Parra Cordero4* and Mercedes N. Lopez1, 2, 3, 5* 1 Faculty of Medicine, University of Chile, Chile 2 Institute of Biomedical Sciences, Faculty of Medicine, Chile 3 Millennium Institute on Immunology and Immunotherapy, Chile 4 Clinical Hospital, Universidad de chile, Obstetrics, Chile 5 Clinical Hospital, Universidad de chile, Chile Introduction: Pregnancy is characterized by various physiological changes in women, promoting a series of anatomical, metabolic, endocrine, and immunological changes that occur during the 9 months of gestation. In a normal pregnancy, there is a balance of Th2 over Th1/Th17 profiles that favors an immune-tolerant environment in order to prevent fetal rejection. Early alterations during the first and second trimester of pregnancy in the imbalance Th1/Th17 v/s Th2 lymphocytes and NK cells may be associated with conditions such as preeclampsia or other pregnancy pathological conditions. Preeclampsia is characterized by hypertension, proteinuria and an exacerbated activation of the immune system in response to altered spiral arteries remodeling due to partial extra-villous trophoblast invasion, which can be assessed non-invasively by uterine artery Doppler throughout pregnancy. The aim of this study was to characterize longitudinally the immune pattern of normal pregnancies according to non-invasive uterine artery assessment. Material and Methods: We measured the expression of inflammatory cytokines IFN-g+, IL-17A+ and IL-4+ in T cells and innate cells like CD3- CD16- CD56 Bright (Regulatory NK cells), CD3- CD16+ CD56+ (peripheral NK Cells), additionally in this cells we evaluated NKp46 (Citotoxicity receptor) from peripheral blood mononuclear cells (PBMC) of fresh or frozen samples obtained from pregnant women at 12, 22 and 32 weeks of gestation using flow cytometry. These pregnancies were sub-classified according to uterine artery Doppler assessment as “high-risk” or “low-risk” for developing preeclampsia. Results: A sub-group of pregnancies showed an increased level of IFN-g and IL-4 producing T cells and increased level of Regulatory NK cells, and NK cell with higher level of NKp46 at 12 weeks compared to 22 &32 weeks of gestation which appears to be associated with uterine artery resistance at the time of sampling. Discussion: These findings might contribute to a better understanding of the role of T cells in normal and pathological pregnancies. Acknowledgements Supported by grants FONDECYT 1130668 & 1130324; IMII P09/016-F. Keywords: Pregnancy Complications, Preeclampsia, natural killer (NK) cells, T lymphocytes, Interleukin-4, Interferon-gamma Conference: IMMUNOCOLOMBIA2015 - 11th Congress of the Latin American Association of Immunology - 10o. Congreso de la Asociación Colombiana de Alergia, Asma e Inmunología, Medellin, Colombia, 13 Oct - 16 Oct, 2015. Presentation Type: Poster Presentation Topic: Immunology of reproduction Citation: Tempio Sepúlveda FI, Rivera Arcos C, Caro Díaz GA, Parra Cordero M and Lopez MN (2015). LONGITUDINAL CHARACTERIZATION OF LYMPHOCYTES OBTAINED FROM PREGNACIES AS LOW OR HIGH RISK OF PREECLAMPSIA. Front. Immunol. Conference Abstract: IMMUNOCOLOMBIA2015 - 11th Congress of the Latin American Association of Immunology - 10o. Congreso de la Asociación Colombiana de Alergia, Asma e Inmunología. doi: 10.3389/conf.fimmu.2015.05.00101 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 15 May 2015; Published Online: 14 Sep 2015. * Correspondence: MD. Mauro Parra Cordero, Clinical Hospital, Universidad de chile, Obstetrics, Santiago, Chile, mcparra@hcuch.cl MD, PhD. Mercedes N Lopez, Faculty of Medicine, University of Chile, Santiago, Chile, melopez@me.com Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Fabián I Tempio Sepúlveda Cristina Rivera Arcos Gustavo A Caro Díaz Mauro Parra Cordero Mercedes N Lopez Google Fabián I Tempio Sepúlveda Cristina Rivera Arcos Gustavo A Caro Díaz Mauro Parra Cordero Mercedes N Lopez Google Scholar Fabián I Tempio Sepúlveda Cristina Rivera Arcos Gustavo A Caro Díaz Mauro Parra Cordero Mercedes N Lopez PubMed Fabián I Tempio Sepúlveda Cristina Rivera Arcos Gustavo A Caro Díaz Mauro Parra Cordero Mercedes N Lopez Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.