Abstract
The role of intra-peritoneal mediators in the regulation peritoneal transport is not completely understood. We investigate the relation between longitudinal changes in dialysis effluent level of nuclear factor kappa-B (NF-κB) downstream mediators and the change in peritoneal transport over 1 year. We studied 46 incident PD patients. Their peritoneal transport characteristics were determined after starting PD and then one year later. Concomitant dialysis effluent levels of interleukin-6 (IL-6), cyclo-oxygenase-2 (COX-2) and hepatocyte growth factor (HGF) are determined. There were significant correlations between baseline and one-year dialysis effluent IL-6 and COX-2 levels with the corresponding dialysate-to-plasma creatinine level at 4 hours (D/P4) and mass transfer area coefficient of creatinine (MTAC). After one year, patients who had peritonitis had higher dialysis effluent IL-6 (26.6 ± 17.4 vs 15.1 ± 12.3 pg/ml, p = 0.037) and COX-2 levels (4.97 ± 6.25 vs 1.60 ± 1.53 ng/ml, p = 0.007) than those without peritonitis, and the number of peritonitis episode significantly correlated with the IL-6 and COX-2 levels after one year. In contrast, dialysis effluent HGF level did not correlate with peritoneal transport. There was no difference in any mediator level between patients receiving conventional and low glucose degradation product solutions. Dialysis effluent IL-6 and COX-2 levels correlate with the concomitant D/P4 and MTAC of creatinine. IL-6 and COX-2 may contribute to the short-term regulation of peritoneal transport.
Highlights
The semi-permeable peritoneal membrane is the basis of successful PD
There was no significant difference in baseline PD effluent IL-6 (23.0 ± 18.3 vs 23.4 ± 42.2 pg/ml, p = 0.9), COX-2 (143.5 ± 73.1 vs 261.3 ± 316.9 ng/ml, p = 0.2) or hepatocyte growth factor (HGF) levels (2.67 ± 1.94 vs 3.56 ± 6.09 pg/ml, p = 0.6) between patients with and without peritonitis episode in the subsequent one year
We observed that the PD effluent IL-6 and COX-2 levels significantly associated with the concomitant dialysate-to-plasma creatinine level at 4 hours (D/P4) and mass transfer area coefficient of creatinine (MTAC) of creatinine, and the change in these cytokines levels correlates with the corresponding alterations in these peritoneal transport parameters
Summary
The study was approved by The Joint Chinese University of Hong Kong – New Territories East Cluster Clinical Research Ethics Committee (reference number CREC-2016.535). We measured the PD effluent levels of interleukin-6 (IL-6), hepatocyte growth factor (HGF), and cyclooxygenase-2 (COX-2) by commercially available enzyme-linked immunosorbent assays (ELISA). These targets were selected because of their potential impact on peritoneal fibrosis and angiogenesis[9,10,11]. Correlation between PD effluent cytokine levels with peritoneal transport function and clinical parameters was calculated by the Spearman’s rank correlation coefficient. All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional research committee at which the studies were conducted and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards. Written informed consent was obtained from all individual participants included in the study
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
