Longitudinal analysis of molecular alteration in serum samples of dextran sodium sulfate-induced colitis mice by using infrared spectroscopy

Abstract

Established diagnostic regimens for inflammatory bowel diseases, such as colonoscopy, sigmoidoscopy, and small bowel follow-through have high sensitivity, but adherence rates of the eligible population for the disease screening is poor due to associated expenses, the risk of complications, time, and comfort issues. Thus, it is critical to develop an Affordable, Sensitive, Specific, User-friendly, Rapid, Equipment-free, and Deliverable (ASSURED) screening test that could increase the participation rate of the eligible group. In this study, we present infrared spectroscopy of serum accompanied with data analysis to monitor longitudinal analysis of molecular alteration in colitis mouse samples. Dextran Sodium Sulfate-induced colitis mouse model sera were used to understand the disease aggravation by testing of the alteration of carbohydrates and the protein secondary structures reflected in the spectra. Samples derived from mouse models at days 0, 3, and 7 of chemical administration were used for spectral measurements. Spectral data at Biomarker peaks show there is not any specific alteration of serum sample composition at day 3 of the chemical feeding, but a significant alteration on the marker can be seen at day 7. The results of this study will thus pave the way for the development of an ASSURED prototype that can have clinical application.