Long-term Visna virus infection of sheep choroid plexus cells: initiation and preliminary characterization of the carrier cultures.

Abstract

Two sheep choroid plexus cell cultures were maintained and propagated for 413 days since being infected with strain K796 visna virus. The majority of the cells in these cultures contained visna virus-specific antigen on days 93 and 105 after infection. Reverse transcriptase-like activity similar to that present in visna virus preparations was obtained from these cultures when very little plaque-forming virus was being synthesized. The persistently infected cultures are resistant to the cytopathic effect which occurs in uninfected cultures upon exposure to visna virus. Persistently infected cells require more time than uninfected cells to become confluent. Less than 0.02 percent of the persistently infected sheep choroid plexus cells form macroscopic colonies within 14 days, whereas 20 to 30 percent of the cells from uninfected cultures form macroscopic colonies within this time.