Long-term vegetation restoration promotes lignin phenol preservation and microbial anabolism in forest plantations: Implications for soil organic carbon dynamics

Abstract

Vegetation restoration contributes to soil organic carbon (C; SOC) sequestration through the accumulation of plant and microbial residues, but the mechanisms underlying this microbially mediated process are not well resolved. To depict the dynamics of plant- and microbial-derived C in restored forest ecosystems, soil samples were collected from Robinia pseudoacacia plantations of different stand ages (15, 25, 35, 45 years old) established on degraded wheat fields. The results showed that the degree of lignin phenol oxidation decreased with increasing stand age (P < 0.05), and hemicellulose-degrading genes were detected at higher relative abundances than other functional gene categories, indicating selective preservation of recalcitrant lignin phenols. Despite both glucosamine (R2 = 0.61, P < 0.001) and muramic acid (R2 = 0.37, P < 0.001) contents trending upward over time, fungal residual C accounted for a greater proportion of SOC compared with bacterial residual C. Accordingly, fungal residual C, which exhibited a similar response pattern as total microbial residual C to vegetation restoration, was considered a major contributor to the SOC pool. These results provided evidence that long-term vegetation restoration enhanced SOC sequestration in R. pseudoacacia forest by promoting the preservation of plant-derived lignin phenols and concomitant microbial anabolism. Partial least squares-discriminant analysis identified two important ecological clusters (i.e., modules) in the fungal network that profoundly influenced lignin phenol oxidation (P < 0.05) and microbial residual C accumulation (P < 0.01). Among the dominant taxa in microbial networks, the bacterial phyla Proteobacteria and Acidobacteriota had potential to degrade recalcitrant C compounds (e.g., cellulose, lignin), whereas the fungal phylum Ascomycota could outcompete for labile C fractions (e.g., dissolved organic C). Findings of this study can enable a mechanistic understanding of SOC stability driven by microbial turnover in restored forest ecosystems.