Abstract
Animal behavior is reflected by locomotor patterns. To decipher the underlying neural circuitry locomotion has to be monitored over often longer time periods. Here a simple adaptation is described to constrain movement of third instar Drosophila larvae to a defined area and use Frustrated total internal reflection based imaging method (FIM) imaging to monitor larval movements up to 1 h. It is demonstrated that the combination of FIM imaging and long analysis periods facilitates the conduction of food choice assays and provides the means to easily quantify food preferences.
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