Abstract

Regional cortical cerebral blood flow is increased via activation of brain nicotinic acetylcholine receptors. Acute intravenous injection of nicotine increases cortical blood flow, without changing systemic blood pressure in anesthetized rats. Here, we examined whether the nicotine-induced cerebral cortical vasodilation is affected by chronic nicotine treatment. Rats received chronic subcutaneous nicotine (at a low or a high-dose) short-term (1 h) or long-term (14 days). Under urethane anesthesia, blood flow in the frontal cortex, before and after bolus injection of nicotine (0.3–30 µg/kg, i.v.) was measured by laser Doppler flowmetry. The threshold dose of nicotine (3 µg/kg, i.v.) producing vasodilation was not affected by chronic nicotine treatment. However, the vasodilation induced by nicotine at 30 µg/kg was reduced after long-term nicotine treatment (but not after short-term exposure). The degree of reduction was marked and was statistically significant with high-dose (100 µg/kg/h) nicotine; low-dose (33 µg/kg/h) nicotine had a small effect that was not statistically significant. In contrast, the vasodilation in the cortical vessels obtained by hypercapnia (inhalation of 10% CO 2) was not changed by chronic nicotine treatment. The nicotine-induced cortical vasodilation was not influenced by methyllycaconitine, an α7-selective nicotinic antagonist, while it was completely abolished by dihydro-β-erythroidine, an α4β2-preferring nicotinic antagonist. We conclude that long-term nicotine treatment reduces the functional activity of α4β2-like nicotinic receptors that mediate cortical vasodilation.

Talk to us

Join us for a 30 min session where you can share your feedback and ask us any queries you have

Schedule a call

Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.