Abstract
The study of hematopoietic stem cells (HSCs) has been facilitated by the transplantation of bone marrow cell populations into lethally irradiated mice. It is widely known that HSCs have the capacity for long-term and multilineage hematopoietic reconstitution in lethally irradiated hosts. Here, we developed a transplantation model system using clonal ginbuna crucian carp (Carassius auratus langsdorfii) that were exposed to a lethal dose of X-rays. The minimum lethal dose (MLD) of ginbuna was approximately 25Gy, which is lethal due to hematopoietic failure. The transplantation of kidney hematopoietic cells into lethally irradiated ginbuna resulted in the rescue of recipient fish for more than 180 days. We examined the reconstitution activity of head kidney (HK), body kidney (BK), and spleen cells. Transplantation experiments showed that only HK and BK cells had the long-term and multilineage reconstitution activity. These results indicate that teleost HSCs have the ability to fully reconstitute the hematopoietic system in lethally irradiated hosts, and that they are present in HK and BK, but not in spleen. This transplantation model system using clonal ginbuna is useful for studies of in vivo kinetics and functions of HSCs in teleosts.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.