Long-term frequency tuning of local field potentials in the auditory cortex of the waking guinea pig.

Abstract

The goal of our study was to determine the extent of changes in frequency tuning in the auditory cortex over weeks. The subjects were awake adult male guinea pigs (n = 8) bearing electrodes chronically implanted in layers IV-VI of primary auditory cortex. Tuning was determined by presenting sequences of pure tone bursts (approximately 0.97-41.97 kHz, -20 to 80 dB, 100-ms tone duration, 5-ms rise-fall, 800-ms intertone intervals, 1.5-s intersequence interval) either in 0.5-octave steps (n = 5, 14 probes) or 0.25-octave steps (n = 3, 9 probes) delivered to the ear contralateral to recording sites. Tuning curves were determined for local field potentials (LFPs), which were tuned to frequency (negative potential, latency to peak 15-20 ms), repeatedly for up to 27 days (0.5 octave) or 12 days (0.25 octave). Characteristic frequency (CF), best frequency at 10 and 30 dB above absolute threshold (BF10, BF30), threshold (TH), and bandwidth (10 dB above threshold; BW) were measured. Absolute amplitude often decreased across weeks, necessitating normalization of amplitude. However, there were no significant trends in tuning over days for CF, BF10, or BF30 for either the half- or the quarter-octave group. Both groups exhibited random daily variations in frequency tuning, the quarter-octave group revealing larger variations averaging 0.228, 0.211, and 0.250 octave for CF, BF10, and BF30, respectively. Therefore, frequency tuning in waking animals does not exhibit directional drift over very long periods of time. However, daily tuning variations on the order of 0.20-0.25 octave indicate that the peaks of tuning curves (CF, BF) represent a preferred frequency range rather than a fixed frequency.