LONG-TERM EFFECTS ON HEK-293 CELL LINE AFTER CO-CULTURE WITH PORCINE ENDOGENOUS RETROVIRUS

Abstract

Xenotransplantation of pig organs, tissues, and cells bears the risk of interspecies transmission of porcine endogenous retrovirus (PERV). To evaluate the long-term effect of PERV infection on human cells, human embryonic kidney cell line HEK-293 cells were co-cultured with PERV produced by the porcine kidney PK15 cell line for 24 hours and the infected HEK-293 cells were continually cultured for 6 months. PERV-gag, pol gene and gag protein were detected in infected HEK-293 cells by PCR and immunofluorescent staining. PERV from the supernatant of infected HEK-293 cells was same as that from PK15 in morphology. The concentration of reverse transcriptase in the supernatant of infected HEK-293 cells was almost 200 times lower than that of PK15 cells. Except that infected HEK-293 cells doubled a little earlier than the control and infected cells grew in serum-free medium poorly, further study for cell morphology and growth showed no significant difference between infected HEK-293 cells and uninfected control. These results suggested although PERV from PK15 could infect human cells in vitro, there was no significant acute effect attributable to PERV infection on the growth of HEK-293 cells by 6 months culture.