Long-term effects of thiosulfate on the competition between sulfur-mediated bacteria and glycogen accumulating organisms in sulfate-rich carbon-deficient wastewater

Abstract

Sewage nutrient (e.g., nitrogen and phosphorus) biological removal performance is often limited by the deficient carbon source and undesirable glycogen accumulating organisms (GAOs), even in sulfate-containing wastewater. Thiosulfate (S2O32−) as a bioavailable, environmentally-benign, metastable and cost-effective agent has been regarded as electron carriers that induces high sulfur-mediated bacterial activity for nutrient removal from wastewater. In this study, the long-term effects of thiosulfate on the competition between sulfur-mediated bacteria (SMB, including sulfur-reducing bacteria (SRB) and sulfur-oxidizing bacteria (SOB)) and GAOs were explored to further close the gap of our knowledge on the control of GAOs under carbon deficient wastewater. Three reactors were continuously operated for over 100 days and were fed with 200 mg acetate-COD/L and 20 (R1), 50 (R2) and 80 (R3) mg S/L thiosulfate respectively. The results revealed that adding thiosulfate at the beginning of the anoxic phase promoted sulfur metabolism and increased the proliferation of SRB (mainly Desulfobacter) and SOB (mainly Chromatiaceae). Correspondingly, the relative abundance of GAOs (mainly Candidatus_Competibacter) decreased. After the carbon source was reduced, the abundance of GAOs increased and the competitive activity of SRB was weakened, resulting in the reduced sulfate reduction, which could be attributed to the fact that GAOs had a higher carbon source competitiveness than SRB under low carbon source conditions. While SOB maintained a high abundance due to the addition of thiosulfate as an additional electron donor, which enhanced the denitrification efficiency. Additionally, the dominant SOB shifted from Thiobacillus to Chromatiaceae during the long-term operation, indicating that Chromatiaceae had a higher competitive advantage for reduced sulfur (e.g., S2O32−, Polysulfide (Poly-S)) and nitrate compared to Thiobacillus. Furthermore, microbial functional genes revealed that S metabolism was enhanced during long-term operation. The potential mechanism and optimization strategy regarding the competition between sulfur-mediated bacteria and GAOs were revealed.