Abstract
ATTEMPTS to grow normal lymphoid tissue for extended periods of time in a state of morphological and immunological differentiation have been met by failure1,2. An alternative procedure involves short-term incubation of lymph node or spleen cells from previously immunized donors3–7. A short time ago Ioachim8 reported successful long-term cultures of normal splenic tissue with continuous production of plasma cells from several mammalian species, including man. Simultaneous attempts with lymph node explants were unrewarding. On the other hand, continuous in vitro growth of mouse lymphomatous cells has been repeatedly accomplished9–11. Recently, Epstein et al.12 reported long-term cultivation of Burkitt lymphoma cells.
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