Abstract
The ex situ conservation of seeds in cryobanks demands previous studies of dehydration procedures for the adjustment of the adequate moisture that makes long-term conservation possible. The objective of this work was to evaluate the effect of dehydration time on the regenerative capacity of embryonic axes of two genipap accessions (Umbaúba and Núcleo Bandeirante). Seeds were submitted to desiccation in policarbonate box containing silica gel for 0, 12, 16 and 20 h at room temperature and after each period, seed moisture content was determined. Seeds were inoculated in germination medium and samples were inserted into cryotubes and immersed in liquid nitrogen (-196ºC). Desiccation in silica gel was efficient in reducing the moisture content in both accessions. Umbaúba accession presented 100% germination at all desiccation treatments before cryopreservation and Núcleo Bandeirante was more sensitive to desiccation with 10% germination in 20 h. Umbaúba accession showed a reduction of growth variables with increased desiccation time. The treatments tested did not affect the growth variables of Núcleo Bandeirante accession. The desiccation, although changes observed in the ultra-structures of embryonic axis, did not influence the regeneration percentage of cryopreserved accessions.
Highlights
Genipap (Genipa americana L.), a native species belonging to Rubiaceae family, is found in northern South America and Central America
The dehydration in silica gel can be applied to genipap accessions, considering that exposure to NL did not compromise the survival and regeneration of embryonic axes from Umbaúba and Núcleo Bandeirante seed accessions
Desiccation in silica gel is efficient in reducing the moisture content (% mc) of Umbaúba and Núcleo Bandeirante seed accessions
Summary
Genipap (Genipa americana L.), a native species belonging to Rubiaceae family, is found in northern South America and Central America. Genipap, as it is popularly known, has potential for socioeconomic use and is used for various purposes, among them for food and textile industrials, wood exploitation and medicines. For presenting resistance to soaked soils, it arouses great interest for the recovery of degraded areas, permanent preservation areas, among others [7, 23]. The species has fruits widely appreciated in the Northeastern region of Brazil, and despite the great demand in the region, it is still exploited in an extractive way [30].
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