Long-term atmospheric CO2 enrichment impact on soil biophysical properties and root nodule biophysics in chickpea (Cicer arietinum L.)

Abstract

Abstract Impact of atmospheric CO 2 enrichment on soil aggregation, carbon and other nutrient availability and soil enzyme activities in relation to root and nodule biophysics in chickpea ( Cicer arietinum L.; Pusa-1105 genotype) was studied in an open top chamber experiment at IARI, New Delhi, India, continuing since 2003. Soil samples were collected at the end of the crop growing seasons of 2010–11 and 2011–12, and analyzed. Root growth and nodulation were also studied in these seasons. Soil C and P pools, and associated enzyme activities responded differently to CO 2 enrichment, while total soil N did not change. Soil labile C fractions viz., water soluble carbohydrate (WSC) and microbial biomass C (MBC) significantly increased, although recalcitrant C fraction declined marginally. The soil-CO 2 flux increased by 28%. Dehydrogenase and fluorescein diacetate hydrolysis activity in soil increased by 44% and 67% respectively; and the β-glucosidase activity enhanced by 20% under enriched CO 2 condition. The CO 2 enrichment induced root growth and N 2 -fixation by root nodules, which were evidenced by increase in legheamoglobin content and nitrogenase activity. Nodules were bulky and had higher starch and soluble sugar contents under enriched atmospheric CO 2 condition, allowing for greater N 2 -fixation. The rhizosphere C:N ratio, however, remained unaffected. It could be possible that larger partitioning of C to roots along with greater N 2 -fixation by nodules in chickpea might stabilize the net C:N ratio in the soil. Moreover, the increased soil biological activity under CO 2 enrichment resulted in marginal depletion of soil recalcitrant C with increase in labile C pools. These are likely to offset the stability of soil C pools in a legume-based agroecosystems under the enriched CO 2 condition in the semi-arid climate.