Long-Term Antibacterial Film Nanocomposite Incorporated with Patchouli Essential Oil Prepared by Supercritical CO2 Cyclic Impregnation for Wound Dressing.

Jingfu Jia,Lifang Sun,Shulei Duan,Xue Zhou,Fahuan Ge,Chengyuan Qin,Ming Li

doi:10.3390/molecules26165005

Jingfu Jia, Lifang Sun + Show 5 more

Open Access

https://doi.org/10.3390/molecules26165005

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Abstract

Biocompatible skin wound dressing materials with long-term therapeutic windows and anti-infection properties have attracted great attention all over the world. The cooperation between essential oil and non-toxic or bio-based polymers was a promising strategy. However, the inherent volatility and chemical instability of most ingredients in essential oils make the sustained pharmacological activity of essential oil-based biomaterials a challenge. In this study, a kind of film nanocomposite loaded with patchouli essential oil (PEO-FNC) was fabricated. PEO-loaded mesoporous silica nanoparticles (PEO-MSNs) with drug load higher than 40 wt% were firstly prepared using supercritical CO2 cyclic impregnation (SCCI), and then combined with the film matrix consisting of polyvinyl alcohol and chitosan. The morphology of PEO-MSNs and PEO-FNC was observed by transmission and scanning electron microscope. The mechanical properties, including hygroscopicity, tensile strength and elongation at break (%), were tested. The release behavior of PEO from the film nanocomposite showed that PEO could keep releasing for more than five days. PEO-FNC exhibited good long-term (>48 h) antibacterial effect on Staphylococcus aureus and non-toxicity on mouse fibroblast (L929 cells), making it a promising wound dressing material.

Highlights

Each year, numerous patients suffer from a variety of skin injuries such as burns, ulcers, fungal infections and other associated traumas, followed with a long recovery period
For the curves of Patchouli essential oil (PEO)-MSNs (Figure 1B), the adsorption/desorption volume reduced significantly compared to that of MSNs, indicating that PEO was successfully loaded into the MSNs
MSNs had a high BET specific surface area value of 610.67 m2 /g, and the BJH adsorption and desorption values including surface area, pore volume and pore width were approximate, demonstrating the well-structured inner mesoporous space. Such large pore volume endowed the MSNs with outstanding drug loading capacity. It was quite different for PEO-MSNs that the BET surface area dropped sharply to 250.18 m2 /g, attributing to the loaded PEO

Summary

Introduction

Numerous patients suffer from a variety of skin injuries such as burns, ulcers, fungal infections and other associated traumas, followed with a long recovery period. An ideal wound dressing requires properties like being non-toxic, good biocompatibility, physical protection, good water absorption and moisture, air permeability, moderate fitting without frequent opening or dressing, stable mechanical properties and producibility of different shapes and sizes [11]. Further combination of different natural polymer materials can effectively improve the mechanical properties and moisture performance of the dressing [14]. The addition of antibacterial drugs into the dressing materials, such as antibiotics, can improve its antibacterial performance [15,16], but how to maintain the long-term antibacterial function of the dressing, reduce the frequency of dressing replacement and reduce the pain of patients, remains an important topic in the treatment of skin diseases. It should be noted that excessive or improper use of antibiotics may lead to the emergence of drug-resistant bacteria and increase the difficulty of treatment [17]

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Conclusion