Abstract
Response Surface Methodology (RSM) was used to assess the optimal conditions for a Water/Oil/Water (W/O/W) emulsion for encapsulated nisin (EN). Nano-encapsulated nisin had high encapsulation efficiencies (EE) (86.66 ± 1.59%), small particle size (320 ± 20 nm), and low polydispersity index (0.27). Biodegradable polyvinyl alcohol (PVA) and polyacrylate sodium (PAAS) were blended with EN and prepared by electrospinning. Scanning electron microscopy (SEM) revealed PVA/PAAS/EN nanofibers with good morphology, and that their EN activity and mechanical properties were enhanced. When the ultrasonication time was 15 min and 15% EN was added, the nanofibers had optimal mechanical, light transmittance, and barrier properties. Besides, the release behavior of nisin from the nanofibers fit the Korsemeyer–Peppas (KP) model, a maximum nisin release rate of 85.28 ± 2.38% was achieved over 16 days. At 4 °C, the growth of Escherichia coli and Staphylococcus aureus was inhibited for 16 days in nanofibers under different ultrasonic times. The application of the fiber in food packaging can effectively inhibit the activity of food microorganisms and prolong the shelf life of strawberries, displaying a great potential application for food preservation.
Highlights
Nowadays, microbial pollution has become a major global public health problem
At 25 ◦C, 5 mg of nanofiber was dispersed in 10 mL phosphate-buffered saline (PBS), placed in a dialysis membrane immersed in 200 mL PBS, and continuously stirred at 400 rpm
0.10 mL bacterial cells were coated on the surface of tryptone soybean agar (TSA) medium, and three nanofiber discs were placed on the inoculation surface
Summary
Microbial pollution has become a major global public health problem. It is an important research direction to add antibacterial agents into basic materials to prepare new materials with antibacterial effects. Stable nisin microcapsules with excellent antimicrobial performance were prepared by response surface methodology (RSM) based on a Box–Behnken design. The microencapsulation of nisin prepared by gelatin encapsulation can effectively protect nisin and has broad development prospects in the food industry. Han et al prepared triaxial fiber membranes containing nisin by electrospinning [4] Their antimicrobial activity persisted for 7 d. To the best of our knowledge, no studies have attempted to use RSM to optimize encapsulated nisin (EN) with maximum EE or prepare antimicrobial PVA/PAAS nanofibers containing EN. The aim of this work is to develop a new antibacterial nanofiber material by electrospinning technology, which is doped with nisin nanoparticles, and is expected to be used as active food packaging
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
