Abstract
A novel biosensor for the highly sensitive detection of aflatoxin M1 (AFM1) in milk was developed. This biosensor is based on surface plasmon-enhanced fluorescence spectroscopy (SPFS) which was advanced through the excitation of long range surface plasmons (LRSPs). In SPFS, the binding of fluorophore-labeled molecules to the sensor surface is probed with surface plasmons (SPs) and the emitted fluorescence light is detected. This approach takes advantages of the enhanced intensity of electromagnetic field occurring upon the resonant excitation of SPs which directly increases the fluorescence signal. For the detection of AFM1, LRSP-enhanced fluorescence spectroscopy was combined with an inhibition immunoassay in which a derivative of AFM1 was immobilized on the sensor surface and antibodies against AFM1 were used as recognition elements. The developed biosensor allowed for the detection of AFM1 in milk within 53min at concentrations as low as 0.6pgmL−1. The achieved limit of detection was about two orders of magnitude lower than the maximum AFM1 residue level in milk stipulated by the European Commission legislation.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
