Abstract
Materials and Methods Relative expression of lncRNAs CARMN, LUCAT1, SMILR, and MALAT1 was tested in clinical aortic tissue and blood plasma samples from TAA and non-TAA patients using the qRT-PCR method. The Mann–Whitney U test was used to compare ΔCt values between the study groups. ROC curve analysis was performed to evaluate the diagnostic value of plasma lncRNAs. Results We found significantly reduced CARMN (p = 0.033) and LUCAT1 (p = 0.009) expression in aortic tissue samples from TAA patients. Relative expression of MALAT1 (p = 0.117) and SMILR (p = 0.610) did not differ in aortic tissue between the TAA and non-TAA groups. Expression of both LUCAT1 and SMILR was significantly decreased in TAA patients' blood plasma compared to controls (p = 0.018 and p = 0.032, respectively). However, only LUCAT1 showed the ability to discriminate aneurysmal disease in patients' blood plasma (AUC = 0.654, 95%CI = 0.534‐0.775, p = 0.018). Conclusions We have shown that the expression of lncRNAs CARMN and LUCAT1 is reduced in dilated aortic tissue and that the LUCAT1 and SMILR expression is lower in the blood plasma of TAA patients. Decreased LUCAT1 expression in TAA patients' blood plasma may have diagnostic potential in discriminating patients with TAA.
Highlights
Thoracic aortic aneurysm (TAA) is mainly an asymptomatic disease with an increasing incidence rate [1]
Aortic tissue samples (N = 24) and blood plasma samples (N = 40) were obtained from sporadic TAA patients, who were diagnosed with aortic aneurysm in accordance with 2014 ESC guidelines on the diagnosis and treatment of aortic disease [19]
Development of thoracic aorta aneurysm is a complex biological process involving smooth muscle cell phenotypic transition. Long noncoding RNAs (lncRNAs) cardiac mesoderm enhancerassociated lncRNA (CARMN), lung cancer-associated transcript 1 (LUCAT1), metastasis-associated lung adenocarcinoma transcript 1 (MALAT1), and smooth muscleinduced lncRNA (SMILR) are associated with the vascular smooth muscle cell (VSMC) phenotypic state in vitro
Summary
Thoracic aortic aneurysm (TAA) is mainly an asymptomatic disease with an increasing incidence rate [1]. Long noncoding RNAs (lncRNAs) involved in VSMC phenotypic regulation could be considered as potential diagnostic indicators and therapeutic targets of TAA. Relative expression of lncRNAs CARMN, LUCAT1, SMILR, and MALAT1 was tested in clinical aortic tissue and blood plasma samples from TAA and non-TAA patients using the qRT-PCR method. Relative expression of MALAT1 (p = 0:117) and SMILR (p = 0:610) did not differ in aortic tissue between the TAA and non-TAA groups Expression of both LUCAT1 and SMILR was significantly decreased in TAA patients’ blood plasma compared to controls (p = 0:018 and p = 0:032, respectively). We have shown that the expression of lncRNAs CARMN and LUCAT1 is reduced in dilated aortic tissue and that the LUCAT1 and SMILR expression is lower in the blood plasma of TAA patients. Decreased LUCAT1 expression in TAA patients’ blood plasma may have diagnostic potential in discriminating patients with TAA
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