Abstract

Objective To explore the biological function and mechanism of lncRNA XIST in breast cancer. Method QRT-PCR was used to detect the expression of lncRNA XIST in breast cancer tissues.The cell viability was tested by CCK-8 after up-regulate XIST.The apoptosis of breast cancer cells was detected by AO/EB.Wound healing and trans well were used to detect the invasion and migration of XIST in ovarian cancer cells.Bioinformatics and Luciferace assay was used to determine the potential targeting effect of XIST. Results XIST was significantly down-regulated in breast cancer tissues and cell lines(P<0.05). Further functional analysis indicated that overexpression of XIST remarkably inhibited breast cancer cell growth, migration, and invasion.The results of luciferase reporter assays verified that miR-1269b was a direct target of XIST in breast cancer.Moreover, SOX6 was identified as a direct target of miR-1269b and miR-1269b/SOX6 rescued the effects of XIST in breast cancer cells. Conclusion XIST is down-regulated in breast cancer and suppresses breast cancer cell growth, migration, and invasion via the miR-1269b/SOX6 axis. Key words: lncRNA XIST; Breast cancer; Migration; Invasion

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