Long non-coding RNA XIST as a potential prognostic biomarker in human cancers: a meta-analysis.

Shaopu Hu,Yimian Li,Yongjun Wang,Edward C Zou,Mengyao Guo,Wenyi Wang,Yanping Yang,Junli Chang

doi:10.18632/oncotarget.23744

Abstract

Growing studies have confirmed that long non-coding RNAs (lncRNAs) involve in the occurrence and development of various cancers. XIST, as a lncRNA, was dysregulated in different cancers. This meta-analysis was performed to evaluate the prognostic potential of XIST in malignant tumors. Eight databases of PubMed, Web of Science, Embase, Cochrane library, CNKI, VIP, SinoMed and Wang Fang were comprehensively searched from their initiation date to August 15, 2017. A total of nine studies with 853 cancer patients met the including criteria were finally included in this meta-analysis after independently screening the literatures by two researchers. Any discrepancies were resolved by a consensus. Hazard ratios (HRs) with corresponding 95% confidence intervals (CIs) for the primary endpoints were extracted and pooled for meta-analysis. Our results showed that expression level of XIST was markedly associated with overall survival (function as oncogene, HR = 0.53, 95% CI: 0.42–0.68, p < 0.00001; function as tumor suppressor, HR = 2.25, 95% CI: 1.15–4.37, p = 0.02), disease free survival (DFS)(HR = 0.45; 95% CI: 0.31–0.67, p < 0.0001), tumor type (digestive system carcinoma, HR = 0.50; 95% CI: 0.37–0.69, p < 0.00001; non-digestive system carcinoma, HR = 0.58; 95% CI: 0.39–0.87, p = 0.008), lymph node metastasis (OR = 0.32, 95% CI: 0.20–0.52, p < 0.00001), distant metastasis (OR = 0.36, 95% CI: 0.22–0.60, p < 0.0001) and tumor stage (OR = 0.43, 95% CI: 0.31–0.60, p < 0.00001). In conclusion, the pooled results in our current work suggest that XIST is an important prognostic biomarker in cancer patients.

Highlights

With the development of deep sequencing methodologies, the function of non-coding RNAs have attracted a wide attention
Our results showed that expression level of X-inactive specific transcript (XIST) was markedly associated with overall survival, disease free survival (DFS)(HR = 0.45; 95% confidence intervals (CI): 0.31–0.67, p < 0.0001), tumor type, lymph node metastasis (OR = 0.32, 95% CI: 0.20–0.52, p < 0.00001), distant metastasis (OR = 0.36, 95% CI: 0.22–0.60, p < 0.0001) and tumor stage (OR = 0.43, 95% CI: 0.31–0.60, p < 0.00001)
LncRNA HULC expression was up-regulated in osteosarcoma tissues and cell lines, and the higher expression of HULC was associated with poor prognosis of osteosarcoma patients

Summary

Introduction

With the development of deep sequencing methodologies, the function of non-coding RNAs (ncRNAs) have attracted a wide attention. The value of these so called “dark matter” was used to be underestimated immensely due to ncRNAs were considered not involved in the encoding proteins. Accumulating reports indicated that lncRNAs were dysregulated in various types of cancers, including breast cancer [3, 4], colon cancer [5], hepatocellular carcinoma [6], gastric cancer [7, 8], osteosarcoma [9, 10] and so on. The dysregulation of lncRNAs is associated with tumor progression and the prognosis of cancer patients

Methods

Results

Conclusion