Abstract
It is generally known that the human genome makes a large amount of noncoding RNAs compared with coding genes. Long non-coding RNAs (lncRNAs) which composed of more than 200 nucleotides have been described as the largest subclass of the non-coding transcriptome in human noncoding RNAs. Existing research shows that lncRNAs exerted biological functions in various tumors via participating in both oncogenic and tumor suppressing pathways. The previous studies indicated that lncRNA taurine upregulated 1 (TUG1) play important roles in the initiation and progression of malignancies. In this study,based on previous research, we investigated the expression and biological role of the lncRNA-TUG1. We analyzed the relationship between lncRNA-TUG1and endometrial carcinoma (EC) in a total 104 EC carcinoma specimens, compared with that in normal tissues. We found that lncRNA-TUG1 expression in cancer tissues was significantly higher than that in adjacent tissues. Through a series of experiments, the results demonstrated that lncRNA-TUG1 enhances the evolution and progression of EC through inhibiting miR-299 and miR-34a-5p.
Highlights
Endometrial carcinoma (EC), the most common form of gynecological malignancy, which can be divided into two types: type I, estrogen-dependent endometriosis carcinoma; and type II, estrogen-independent nonendometriosis carcinoma [1,2,3]
Similar results were obtained with the ishikawa cell line, 68.6% Long non-coding RNAs (lncRNAs)-taurine upregulated 1 (TUG1) was detected in the cytoplasmic fraction, and 34.8% was situated in the nuclear fraction (Figure 1B)
We detected the lncRNA-TUG1 expression in endometrial carcinoma (EC), the results showed that it was dramatically up-regulated in EC tissues and we further explored functional role and possible mechanism of lncRNA-TUG1 in EC
Summary
Endometrial carcinoma (EC), the most common form of gynecological malignancy, which can be divided into two types: type I, estrogen-dependent endometriosis carcinoma; and type II, estrogen-independent nonendometriosis carcinoma [1,2,3]. LncRNAs as a competing endogenous RNA (ceRNA) and sponge miRNAs, regulating the expression of target mRNA is becoming a new hotspot of epigenetics research [9]. LncRNA TUG1 was frequently upregulated and characterized as an oncogene involved in the development and progression of a variety of tumors (e.g., osteosarcoma [11], esophageal squamous cell carcinoma (ESCC) [12], urothelial carcinoma of the bladder [13], glioblastoma [14], colorectal cancer [15]). Previous studies indicated that lncRNA TUG1 through regulate the expression of the miRNA and its target genes involved in the development and progression of cancer. We delineate the transcriptional aberration of lncRNA TUG1 in EC tumor tissues and corresponding adjacent normal tissues www.impactjournals.com/oncotarget
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