Long Noncoding RNA SNHG1 Activates Autophagy and Promotes Cell Invasion in Bladder Cancer.

Changcheng Guo,Xudong Yao,Yang Yan,Jiang Geng,Xin Li,Dan Liu,Jinbo Xie,Lin Ye,Ming Luo

doi:10.3389/fonc.2021.660551

Abstract

LncRNAs play important roles in bladder cancer. However, only a few studies report on the correlation between lncRNAs expression and autophagy in bladder cancer. This study aimed to explore the effect of lncRNA on autophagy in bladder cancer. The findings showed high expression of SNHG1 in the bladder cancer cells and tumor tissues. The high expression of SNHG1 was positively correlated with bladder cancer cell invasion, proliferation, and autophagy. This finding implies that SNHG1 promotes bladder cancer cell invasion and proliferation via autophagy. Further analysis of the mechanism of action of SNHG1 showed that it functions as a sponge of miRNA-493 in bladder cancer. miRNA-493 binds on the 3’ -UTR of ATG14 mRNA thus affecting ATG14 protein expression, which is implicated in autophagy. These findings are supported by previous preclinical studies using multiple Bca cell lines and TCGA, which demonstrate that SNHG1 plays an oncogenic role by acting as a sponge of miR-493-5p or as its ceRNA. Upregulation of SNHG1 promotes proliferation, invasion, and autophagy of bladder cancer cells through the miR-493-5p/ATG14/autophagy pathway. Therefore, SNHG1 may act as a potential therapeutic target for the treatment of bladder cancer.

Highlights

Bladder cancer (Bca) is the second most common malignancy of the urinary tract
SNHG1 expression in T24, RT4, RT112, 253J, and DSH1 bladder cancer cells was determined and the results showed high expression levels of SNHG1 in all the bladder cancer cells
The findings indicated that high expression levels of SNHG1 in bladder cancer patients are associated with a short survival time (Figure 1C)

Summary

Introduction

Bladder cancer (Bca) is the second most common malignancy of the urinary tract. There are approximately 2 million bladder cancer patients worldwide [1]. In China, bladder cancer is the most common urologic neoplasm, with 80, 500 new cases and 32, 900 deaths reported in 2015 [3]. Current treatment approaches are mainly effective in patients with non-metastatic bladder cancer. The survival rate of patients with metastatic bladder cancer is poor [6]. Diagnosis and treatment play an important role in improving the SNHG1 Activates Autophagy in Bca prognosis of bladder cancer. Studies should explore mechanisms underlying the malignant progression in bladder cancer for the development of effective therapies [3]

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