Abstract
BackgroundDiabetic nephropathy (DN) is a common diabetic complication. Long noncoding RNAs (lncRNAs) have been identified as essential regulators in DN progression. This study is devoted to the research of lncRNA-myocardial infarction-associated transcript (MIAT) in DN.MethodsDN cell model was established by high glucose (HG) treatment for human renal tubular epithelial cells (HK-2). Cell viability and colonizing capacity were analyzed by Cell Counting Kit-8 (CCK-8) and colony formation assay. Apoptosis was assessed via caspase-3 detection and flow cytometry. Enzyme-linked immunosorbent assay (ELISA) was used for evaluating inflammation. The protein determination was completed using western blot. MIAT, microRNA-182-5p (miR-182-5p), and G protein-coupled receptor class C group 5 member A (GPRC5A) levels were all examined via reverse transcription-quantitative polymerase chain reaction (RT-qPCR). Intergenic binding was verified using dual-luciferase reporter, RNA immunoprecipitation (RIP), and RNA pull-down assays.ResultsHG induced the inhibition of cell growth, but accelerated apoptosis and inflammation as well as the activation of nuclear factor kappa B (NF-κB) pathway. MIAT reestablishment prevented the HG-induced cell damages and NF-κB signal activation. Mechanistically, MIAT was proved as a miR-182-5p sponge and regulated the expression of GPRC5A that was a miR-182-5p target. The rescued experiments demonstrated that MIAT downregulation or miR-182-5p upregulation aggravated the HG-induced cell damages and activated the NF-κB pathway via the respective regulation of miR-182-5p or GPRC5A.ConclusionTaken together, MIAT functioned as an inhibitory factor in the pathogenesis to impede the development of DN and inactivate the NF-κB pathway via regulating the miR-182-5p/GPRC5A axis.
Highlights
Diabetic nephropathy (DN), the most familiar microvascular complication in diabetic type 1 and type 2 patients with high incidence rate, is considered as the leading risk factor of chronic kidney failure and end-stage renal diseases [1,2]
A recent study reported that the antagonistic effect of Myocardial infarction-associated transcript (MIAT) on DN progression was related to the sponge function on miR-147a and the expression regulation on E2F3 [16]
1 × 106 high glucose (HG)-treated HK-2 cells were lysed in RNA immunoprecipitation (RIP) lysis buffer and Protein A magnetic beads were pre-coated with antibodies targeting Argonaute-2 or immunoglobulin G
Summary
Diabetic nephropathy (DN), the most familiar microvascular complication in diabetic type 1 and type 2 patients with high incidence rate, is considered as the leading risk factor of chronic kidney failure and end-stage renal diseases [1,2]. MIAT represses the progression of DN 1337 has been correlated to the renal tubular epithelial injury in DN [12], and MIAT could enhance cell viability in high glucose (HG)-treated renal tubular cells by increasing the expression of Nrf2 [15]. A recent study reported that the antagonistic effect of MIAT on DN progression was related to the sponge function on miR-147a and the expression regulation on E2F3 [16]. It is unknown whether the regulation of MIAT in DN progression is related to other molecular mechanism
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