Abstract
Gastric cancer is one of the common causes of cancer mortality worldwide, with a low survival rate for the affected people. Recent studies have revealed the key role of long non-coding RNAs (lncRNAs) in the development and progression of many cancers, including gastric cancer. Looking for the potential molecular regulators of gastric cancer incidence and progression, LINC02381 was identified as a downregulated lncRNA in gastric cancer tissues by analysis of available microarray and RNA-seq data and RT-qPCR confirmed this differential expression. MiR-21, miR-590, and miR-27a miRNAs were predicted to be sponged by LINC02381, and dual luciferase assay verified LINC02381 as a competitive endogenous RNA (CeRNA), which binds to them. Furthermore, we found that increased expression of LINC02381 attenuates Wnt pathway activity. Also, functional analysis indicates that LINC02381 arrests cell cycle, increases apoptosis and caspase activity, and reduces cell survival and proliferation rate of the human gastric cancer cell lines AGS and MKN45. Moreover, EMT analysis showed that LINC02381 is involved in gastric cancer progression and inhibits metastasis. Overall, this work for the first time introduces LINC02381 as a CeRNA involved in gastric cancer and provides novel insight into the molecular pathogenesis of gastric cancer.
Highlights
Gastric cancer is the third leading cause of cancer-related mortality worldwide despite the great progress in the diagnosis and treatment of human malignancies [1, 2]
The GSE79973 experiment was analyzed to identify long noncoding RNAs (lncRNAs) that have the potential to play a role in gastric cancer progression, which were related to the study of gene expression in 10 pairs of gastric cancer samples
The data showed that LINC02381 has a higher basal expression than other lncRNAs and has a distinct expression in gastric tissue compared to other tissues (Figure 1C)
Summary
Gastric cancer is the third leading cause of cancer-related mortality worldwide despite the great progress in the diagnosis and treatment of human malignancies [1, 2]. LncRNAs play roles in modulating a wide range of cellular processes through affecting various aspects of chromatin modification, transcription, and posttranscriptional processing [6, 9]. It is very important for clinical diagnosis and therapy of LOC400043 Regulates Wnt Signaling gastric cancer to explore the biological function of lncRNAs. Recently, an emerging regulatory role of lncRNAs, competitive endogenous RNA (CeRNA), has been proposed. An emerging regulatory role of lncRNAs, competitive endogenous RNA (CeRNA), has been proposed In this way, lncRNAs, by scavenging miRNAs, can reduce the number of miRNAs available for the target mRNA and indirectly prevent the target gene repression [10, 11]. The function of lncRNA as CeRNA has been investigated in several cancers including glioblastoma, colorectal cancer, and gastric cancer [12,13,14,15]
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