Abstract
Long non-coding RNAs (lncRNAs) are a new arm of gene regulatory mechanism as discovered by sequencing techniques and follow-up functional studies. The lncRNAs regulation of pseudorabies virus (PRV) infection has rarely been reported so far. Using RNA sequencing analysis, 225 lncRNAs with significant altered expressions in 3D4/21 cells infected with PRV (ZJ01) were identified. Five lncRNAs upregulated in PRV-infected cells were verified in cells infected with different PRV strains by qRT-PCR. By down- and up-regulation of lnc641, the accelerating effect of lnc641 on PRV replication was confirmed. Furthermore, we found that lnc641 regulated PRV replication by inhibiting the JAK-STAT1 pathway. This study suggests that lnc641 could be a new host factor target for developing antiviral therapies against PRV infection.
Highlights
Pseudorabies virus (PRV), known as Aujeszky’s disease virus or suid herpesvirus 1, is a member of the alphaherpesvirinae subfamily and threatens pig production [1]
LncRNAs are differentially expressed in PRV‐infected 3D4/21 cells To identify Long non-coding RNAs (lncRNAs) that are dysregulated during Pseudorabies virus infection, 3D4/21 cells infected with ZJ01 PRV strain at a MOI of 0.5 for 22 h were submitted to RNA-seq analysis
In addition to proteins encoded by viruses, noncoding RNAs including microRNAs and lncRNAs in host cells may play an important role in viral infections [24,25,26]
Summary
Pseudorabies virus (PRV), known as Aujeszky’s disease virus or suid herpesvirus 1, is a member of the alphaherpesvirinae subfamily and threatens pig production [1]. PRV genome is a double-stranded DNA with a length of 142 334 bp [2, 3]. The mature virion, or infectious viral particle, consists of four morphologically distinct structural components: the central core contains the linear double-stranded DNA genome of the virus; the DNA is enclosed within a protective icosahedral capsid to form a nucleocapsid; the capsid is embedded in a protein matrix known as the tegument; the tegument is surrounded by the envelope, a lipid membrane. Host genome DNA sequencing is of importance for understanding host evolution, disease origin, and the interplay between environment and heredity. Long noncoding RNAs (lncRNAs) constitutes a rich category. Some estimates suggest that the human genome contains more than 90 000 genes and approximately 60 000 of them are lncRNAs, while other estimates suggest that the number of lncRNA genes could reach closer to 200 000 [9]
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