Long Noncoding RNA Lnc-TLN2-4:1 Suppresses Gastric Cancer Metastasis and Is Associated with Patient Survival.

Yuyun Wu,Xin Yang,Shiming Yang,Ningbo Hao,Bosheng Li,Suming Wang,Yufeng Xiao,Huan Yang

doi:10.1155/2020/8681361

Abstract

Gastric cancer (GC) is one of the most common malignancies worldwide, and the tumor metastasis leads to poor outcomes of GC patients. Long noncoding RNAs (lncRNAs) have emerged as new regulatory molecules that play a crucial role in tumor metastasis. However, the biological function and underlying mechanism of numerous lncRNAs in GC metastasis remain largely unclear. Here, we report a novel lncRNA, lnc-TLN2-4:1, whose expression is decreased in GC tissue versus matched normal tissue, and its low expression is involved in the lymph node and distant metastases of GC, as well as poor overall survival rates of GC patients. We further found that lnc-TLN2-4:1 inhibits the ability of GC cells to migrate and invade but does not influence GC cell proliferation and confirmed that lnc-TLN2-4:1 is mainly located in the cytoplasm of GC cells. We then found that lnc-TLN2-4:1 increases the mRNA and protein expression of TLN2 in GC cells and there is a positive correlation between the expression of lnc-TLN2-4:1 and TLN2 mRNA in GC tissue. Collectively, we identified a novel lncRNA, lnc-TLN2-4:1, in GC, where lnc-TLN2-4:1 represses cell migration and invasion. The low expression of lnc-TLN2-4:1 is associated with poor overall survival rates of GC patients. These suggest that lnc-TLN2-4:1 may be a tumor suppressor during GC metastasis.

Highlights

Gastric cancer (GC) is the fifth most common cancer and the third leading cause of cancer mortality worldwide [1]
A recent report showed that Long noncoding RNAs (lncRNAs) GMAN promotes translation of ephrin A1 (EFNA1) mRNA into protein via binding to the antisense GMAN-AS, which is complementary to EFNA1 mRNA, resulting in the enhancing ability of GC cells to metastasize and invade, so that it leads to GC metastasis and poor patient survival [9]
Based on stringent filtering criteria, we found an unidentified lncRNA, AF070527, whose expression was decreased in three GC tissues compared with the matched normal tissues (Figure 1(a)). e name of this lncRNA has been updated to lnc-TLN2-4:1 in LncBook, a curated knowledgebase of human lncRNAs

Summary

Introduction

Gastric cancer (GC) is the fifth most common cancer and the third leading cause of cancer mortality worldwide [1]. E major reason that leads the patient to die is GC metastasize [3], but the underlying mechanism remains largely unclear. Ere are several regulatory mechanisms involved in lncRNAs, such as (1) lncRNAs interact with proteins, resulting the functional change of the proteins or their locations in the cell organs [6]; (2) lncRNAs serve as competitive endogenous RNAs that absorb miRNAs, thereby controlling the expression of miRNAs’ target genes [7]; (3) lncRNAs bind to mRNAs and prevent mRNAs from degradation, or influence their translation [8]. A recent report showed that lncRNA GMAN promotes translation of ephrin A1 (EFNA1) mRNA into protein via binding to the antisense GMAN-AS, which is complementary to EFNA1 mRNA, resulting in the enhancing ability of GC cells to metastasize and invade, so that it leads to GC metastasis and poor patient survival [9].

Methods

Results

Conclusion