Long non-coding RNA LINC01426 facilitates glioblastoma progression via sponging miR-345-3p and upregulation of VAMP8

Jingwei Cao,Zhiqiang Su,Zhanbin Tang

doi:10.1186/s12935-020-01416-3

Abstract

BackgroundLong non-coding RNAs (lncRNAs) has been extensively reported play important roles in regulating the development and progression of cancers, including Glioblastoma (GBM). LINC01426 is a novel lncRNA that has been identified as an oncogenic gene in GBM. Herein, we attempted to elucidate the detailed functions and underlying mechanisms of LINC01426 in GBM.MethodsLINC01426 expression in GBM cell lines and tissues were detected by quantitative real-time PCR (qRT-PCR). Cell Counting Kit-8 (CCK8) assays, colony formation assays, subcutaneous tumor formation assays were utilized to investigate the biological functions of LINC01426 in GBM. Dual-luciferase reporter assays, RNA immunoprecipitation (RIP) and bioinformatic analysis were performed to determine the underlying mechanisms.ResultsLINC01426 is up-regulated in malignant GBM tissues and cell lines and it is capable to promote GBM cell proliferation and growth. Mechanistically, LINC01426 serves as a molecular sponge to sequester the miR345-3p and thus enhancing the level of VAMP8, an oncogenic coding gene, to promote GBM progression.ConclusionsOur results revealed the detailed mechanisms of LINC01426 facilitated cell proliferation and growth in GBM and report the clinical value of LINC01426 for GBM prognosis and treatment.

Highlights

Long non-coding RNAs has been extensively reported play important roles in regulating the development and progression of cancers, including Glioblastoma (GBM)
LINC01426 is highly expressed in GBM and predicts poor prognosis To identify oncogenic Long non-coding RNAs (lncRNAs) involved in GBM progression, we initially selected 20 previous reported cancer-associated lncRNAs (Additional file 1: Fig. S1) and retrieved their expression in the cancer genome atlas (TCGA)GBM patients’ cohort by an online analysis tool GEPIA
We found that lncRNAs including LINC00511, LINC01426, GAS5, HOXA-AS2, CRNDE and DLEU1 are significantly up-regulated in GBM tissues (Additional file 1: Fig. S1)

Summary

Introduction

Long non-coding RNAs (lncRNAs) has been extensively reported play important roles in regulating the development and progression of cancers, including Glioblastoma (GBM). GBM is the most aggressive and common form of brain cancer in adults, it is characterized by poor survival and remarkably high tumors heterogeneity and lack of effective therapies [1, 2]. The major barriers to effective treatment of GBM are their high proliferation, progressive spread, and invasiveness, but the underlying mechanisms for controlling gliomas are still far from understood [5]. There is an urgent need to LncRNAs have been shown play pivotal roles such in tumorigenesis and cancer progression, including prostate [6], colorectal [7], breast [8], bladder [9], liver [10] and brain cancers [11,12,13].

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