Abstract
Breast cancer is the second leading cause of cancer‐related deaths in women. The long noncoding RNA LINC00115 has been reported to be involved in the poor outcome of patients with breast cancer, but the biological function and underlying mechanism remain unclear. Here, we report that LINC00115 expression is increased in triple‐negative breast cancer tissue compared with matched normal tissue, and LINC00115 knockdown suppresses breast cancer cell migration and invasion. Furthermore, we show that LINC00115 directly targets miR‐7 and inhibits its expression. LINC00115 also reduces the expression of KLF4, which is a direct target of miR‐7 and is involved in breast cancer metastasis. Together, our findings suggest that LINC00115 promotes breast cancer metastasis through modulating the expression of miR‐7 and KLF4.
Highlights
Breast cancer is the most common malignancy and the second leading cause of cancer-related deaths in women
We found that LINC00115 expression is increased in Triple-negative breast cancer (TNBC) tissue compared with matched normal tissue, and LINC00115 knockdown inhibits the migration and invasion of breast cancer cells
We detected the expression of LINC00115 in the tissues using quantitative reverse transcription polymerase chain experiments, and the results showed that LINC00115 expression was significantly increased in TNBC tissues compared with matched normal tissues (Fig. 1A), and elevated in lymph node-metastatic TNBC tissues than in nonmetastatic TNBC tissues (Fig. 1B)
Summary
Breast cancer is the most common malignancy and the second leading cause of cancer-related deaths in women. An increasing number of lncRNAs have been reported to participate in Abbreviations lncRNA, long noncoding RNA; qRT–PCR, quantitative reverse transcription polymerase chain; ROC, receiver operating characteristic; TNBC, triple-negative breast cancer. FEBS Open Bio published by John Wiley & Sons Ltd on behalf of Federation of European Biochemical Societies Xu et al [10] integrated the GEO data and TCGA data, as well as their own RNA-sequencing data, and revealed hundreds of oncogenic lncRNAs in breast cancer, among which LINC00115 is involved in the survival time of patients with breast cancer and do not respond to estrogen. We demonstrated that LINC00115 directly represses the expression of miR-7, which has been confirmed to suppress breast cancer metastasis
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