Abstract
BackgroundLong noncoding RNAs (LncRNAs) have been reported to critically regulate gastric cancer (GC). Recently, it was reported that LBX2 antisense RNA 1 (LBX2-AS1) is abnormally expressed in GC. However, the role of LBX2-AS1 in the malignancy of GC is worth further discussion.MethodsQuantitative real-time polymerase chain reaction (qRT-PCR) was used to determine the LBX2-AS1, miR-4766-5p and C-X-C motif chemokine (CXCL5) expression in GC tissues and cells. Dual-luciferase reporter assay was applied to examine the target relationship between LBX2-AS1 and miR-4766-5p or miR-4766-5p and CXCL5. Cell counting kit-8 (CCK-8) and Transwell assays were used to detect cell proliferation, migration and invasion rates. The protein expression of CXCL5 was confirmed using western blot. The RNA pull down experiment was used to verify the specificity of LBX2-AS1 and miR-4766-5p on BGC-823 and SGC-7901 cells.ResultsLBX2-AS1 was up-regulated in GC tissues and cells, and its knockdown suppressed proliferation, migration and invasion of GC cells. While, overexpression of LBX2-AS1 increased proliferation and increased CXCL5 mRNA level. CXCL5 improved cell proliferation, migration and invasion of GC cells. LBX2-AS1 could bind to miR-4766-5p to regulate CXCL5 expression. Overexpression of CXCL5 overturned those effects of miR-4766-5p in GC cells. RNA Pull down shown that BGC-823 and SGC-7901 cells, miR-4766-5p specifically binds to LBX2-AS1.ConclusionsIn short, this study demonstrated that LBX2-AS1 promoted proliferation, migration and invasion through up-regulation CXCL5 mediated by miR-4766-5p in GC. The LBX2-AS1/miR-4766-5p/CXCL5 regulatory axis provides a theoretical basis for the research on lncRNA-directed therapeutics in GC.
Highlights
Long noncoding RNAs (LncRNAs) have been reported to critically regulate gastric cancer (GC)
LBX2‐AS1 was highly expressed in GC tissue and cells In the first, we detected the expression of LBX2-AS1 in GC tissues and cells
LBX2-AS1 expression in three GC cells (MKN-45, BGC823 and SGC-7901) was significantly up-regulated compared with the human gastric mucosal epithelial cell line (GES-1) (Fig. 1b, P < 0.05, respectively)
Summary
Long noncoding RNAs (LncRNAs) have been reported to critically regulate gastric cancer (GC). It was reported that LBX2 antisense RNA 1 (LBX2-AS1) is abnormally expressed in GC. Gastric cancer (GC) is a one of the most common malignant tumors, and its morbidity ranks the first cancer in China [1]. Studies have found that lncRNAs are widely involved in almost all physiological and. LBX2 antisense RNA 1 (LBX2-AS1), transcribed from the intron of chromosome 2p13.1, was a newly discovered lncRNA which was initially reported to act as a tumor promoter [6]. Studies have shown that LBX2AS1indicates poor prognosis and promotes cell proliferation and metastasis through Notch signaling in non-small cell lung cancer [7]. Abnormal expression of LBX2-AS1 is associated with poor prognosis of GC [8]
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