Abstract
Long Noncoding RNAs (lncRNAs) are a kind of non-protein coding transcripts longer than 200 nucleotides, and play important roles in diverse biological processes, such as embryonic development and apoptosis. Homeobox (HOX) transcript antisense intergenic RNA (HOTAIR) is a negative prognostic factor in a variety of human cancers, such as breast, liver and lung cancers. HOTAIR can promote cancer cell metastasis by reprogramming chromatin organization. In the present study, HOTAIR expression was elevated in tissues of renal cell carcinoma compared to adjacent normal tissues, and positively correlated with metastasis (P<0.05). The cell migration was inhibited in scratch test and transwell assay after HOTAIR knockdown (P<0.05). Further researches revealed that histone demethylase JMJD3 was reduced and its target gene Snai1 expression was down-regulated after HOTAIR suppression (P<0.05). Meanwhile, the level of histone methytransferase EZH2 target gene PCDHB5 was increased (P<0.05). Collectively, these data suggest that HOTAIR is an important promoter in metastasis of renal cell carcinoma and also plays a dual regulatory role in chromatin state by effecting both histone metylation and demethylation at different gene loci.
Highlights
Renal cell carcinomas (RCC) is among the 10 most common cancers, which accounts for 2% to 3% of all adult malignancies and causes 100,000 deaths per year worldwide [1]
To understand the biological significance of Long Noncoding RNAs (lncRNAs) Homeobox transcript antisense intergenic RNA (HOTAIR) in RCC development, the mRNA levels of HOTAIR were examined in cancer tissues and corresponding noncancerous tissues from 36 RCC patients using Quantitative real-time polymerase chain reaction (qRT-PCR)
Long Noncoding RNAs (LncRNAs) are emerging as key players in various fundamental biological processes and altered expression of them is associated with tumorigenesis, tumor progression and metastasis [12]
Summary
Renal cell carcinomas (RCC) is among the 10 most common cancers, which accounts for 2% to 3% of all adult malignancies and causes 100,000 deaths per year worldwide [1]. Average survival time for the metastatic RCC was under a year. It is essential for improvement of metastatic RCC therapy to further understand the mechanism of RCC. Many studies have demonstrated that abnormal content or function of lncRNAs are related to tumor formation, progression, and metastasis of many kinds of cancer, such as prostate, bladder, and kidney cancers [4]. It is very important for clinical diagnosis and therapy of diverse cancers to explore the biological function of lncRNAs [5]
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